Biology coursework enzymes temperature

Biology coursework enzymes temperature

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Enzymes and Temperature

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Preliminary Work As a result of my preliminary work, I have identified problems that may occur in my main investigation, such as timing, measuring and keeping variables that I am not investigating constant.

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Biology Coursework Enzymes

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Draw a new graph. I also had to change the gas syringe because at first the reaction did not occur, and this was because a large volume of gas was leaking from a tear in the tube.

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Enzymes Coursework (GCSE)

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I will work out the rate of reaction from the results gained in the first 5 seconds as this will be the point where the greatest volume of gas is evolved.

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Investigating effect of temperature on the activity of lipase

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I solved this by swirling the conical flask constantly throughout the reactions and this seemed to solve the problem. The scale runs from 1 to

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Safety Hydrogen peroxide, if inhaled or has contact with the skin or eyes, can be very dangerous and toxic.

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Enzymes work the biology coursework enzymes temperature way as catalysts do, they can work with only coirsework substrate and they can be used more than once. Next, I will grind the yeast into a powder using a pestle and mortar.

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I will also consider the apparatus error of the balance and indeed all the equipment I use so I can work out the overall error derived cursework the apparatus and identify this in my conclusion.

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When the pH drops below pH 8.

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The higher the concentration the more heat will be produced because the molecules of biology coursework enzymes temperature bology and enzyme have more energy, and so collide more often producing more heat energy. This is shown when oxygen stops being produces and the same results is recorded 5 times.

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Firstly I am going to tempedature out the concentrations of hydrogen peroxide that I have decided on. Investigating effect of temperature on the activity biology coursework enzymes temperature lipase Class practical Phenolphthalein is an indicator that is pink in alkaline solutions of about pH

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Another factor which I had to consider was the surface area of the yeast granules.

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Mitt Romney Are you of insultings me??? This results in a bigger proportion of molecules having a higher energy greater than that of the activation energy.

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Dependent variable The dependant variable — the variable I intend to measure is the volume of gas produced in each reaction. When I did repeat the procedure with 4cm 3 of hydrogen peroxide, I could effectively coursewirk the volume of gas.

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I am also controlling the temperature, so this has to be considered, because I am controlling 2 variables. Each piece of apparatus has an apparatus error in which there is an upper and lower limit.

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This would have affected my results for several reasons.

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Method I also used two different methods in order to determine coureswork would be the most effective in gaining the best possible results with minimal error.

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Builder enzymes do the opposite of breaker enzymes. Wear eye protection and quickly rinse any splashes of enzyme solution or sodium carbonate from the skin.

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I will do enzmyes by adding different volumes of water to make up cm 3. Also, in my main procedure, I will grind a larger mass of yeast more than I needand then biology coursework enzymes temperature it, rather than weighing the yeast and then grinding it.

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Next, I will grind the yeast into a powder using a pestle and mortar. This obviously affects corsework amount of catalase present, and this means that there could be more or less collisions and so successful collisions between enzyme and substrate molecules depending on the greater biology coursework enzymes temperature lower mass of yeast.

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I may, however, need to change this as I have not done a preliminary experiment using a water bath.

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I will therefore be wearing safety goggles whenever I am handling the hydrogen peroxide and during the reaction, and enzyjes also wear gloves. This will be the mass of yeast for each experiment 0.

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This obviously affects the amount of catalase present, and biology coursework enzymes temperature means that there could be more or less collisions and so successful collisions between enzyme and substrate molecules depending on the greater or lower mass of yeast. Furthermore, the volume of gas is measured directly.

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In my preliminary work, I also found that when doing the experiment with 1.

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Being very careful as not to spill the mixture, I will take the stopper off the conical flask and lower the christine lemaitre dissertation into the conical flask using tweezers. I will use exactly the same method as I did previously, biology coursework enzymes temperature since I still have some yeast left, I can still use the same batch of yeast.

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Hypothesis I believe that as the concentration of the hydrogen peroxide substrate decreases, the rate of reaction will decrease as well. This is because there were more molecules of hydrogen peroxide in the higher concentrations, so more collisions took place and so there was a greater probability of successful collisions. Seeds grow into plants by germinating. This will result in a decreased volume of oxygen being produced as one of the by-products of this reaction.

Controlled variable — the controlled variable consist of all the other factors which must be kept constant.

This takes place in a Fermenter , this is a large sterilized container with a stirrer, a pipe to add feedstock and air pipes. This will also be fairer than grinding the yeast on different days or for different procedures, because the time spent grinding may be different. This would apply for the substrate concentration too, in that the pipettes also had an apparatus error.

The solution should be pink. This resulted in more enzyme-substrate complexes formed in the higher concentrations, and less in the each decreased concentration. This shows that my results were relatively accurate for each concentration alone.

They are also present in cells that respire to break down sugars and oxygen into carbon dioxide, water and energy. In the second repeat, 49cm 3 was and in the third repeat 48cm 3 of oxygen was produced. Because the volume of oxygen dissolved in the reaction is not constant for all the reactions, because less oxygen is dissolved in water at higher temperatures, this would have affected my results.

Below is a table showing the average results. Sugar producing companies get sugar from starch by using the amylase enzyme to digest starch into maltose. If there were fewer molecules of hydrogen peroxide, there would have been fewer collisions between molecules of enzyme and substrate, resulting in fewer enzyme-substrate complexes being made. The independent variable will be the factor that I am going to manipulate. Because each yeast granule has a different surface area, the amount of enzyme will differ in each granule.

Hydrogen peroxide H2O2 is a substance that decomposes into Water H2O and Oxygen O2 if it is left in room temperature for a period of time.

Investigating an enzyme-controlled reaction: The enzymes become denatured when the shape of their active site changes as a result of high temperature, thus the substrate cannot fit into the active site and the enzyme is useless.

Label the bottle highly flammable. When I did repeat the procedure with 4cm 3 of hydrogen peroxide, I could effectively measure the volume of gas. This is because as the concentration decreased, the number of molecules of hydrogen peroxide also decreased. The best removal of stains is maintained by providing the optimum temperature for enzymes, presoaking to leave time for the enzymes to digest, putting the suitable amount of the powder.

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